Test up to 3. EMax. Compact designs. The Gemini instruments can read some luminescence but it is about 100 times less sensitive than a dedicated luminometer. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 1. M2. Support. 2. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Absorbance Plate Reader Comparison Chart. 2 Software Release Notes. Cell line development teams will realize savings on time and resources,SpectraMax i3x. Weight. Molecular Devices' microplate readers. Urban myths of microplate readers. com #THX1138 Introduction The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes. 1 up to 3600 sec. #Flex3) FlexStation 3 8-channel Pipettor Head (Molecular Devices cat. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is. “Sensitivity is pretty much clutch for luminescence,” says Daly. Tutorial on how to quickly and easily view your results on the SpectraMax iD3 Multi-Mode Microplate Reader. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. #S1205. 211, and 820 and EudraLex Volume 4 and Annex 11 and 15, and how they apply to the implementation of Molecular Devices microplate readers and SoftMax Pro. Molecular Devices makes. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . 더 많은. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. Shaking. Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. 9. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Materials • Quant-iT PicoGreen dsDNA Assay Kit, including lambda DNA standard (Life Technologies cat. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The sample is typically placed in a cuvette through which light is sent horizontally. Contact Us Request Quote Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please. 8 foot) 4400-0002 Power cord, 1 meter (3. Absorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. #X7060. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. Getting to know your new SpectraMax iD3 Multi-Mode Microplate Reader View a brief overview of the features and benefits of your new SpectraMax iD3 Multi-Mode. Purchase a new SpectraMax Multi-Mode Microplate Reader and. DOCUMENTATION. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. SpectraMax readers support page; Analyst and FlexStation reader support page; Plate handlers and washers support page; We are continuously adding material to the knowledge base. ] Example of document to evaluate if the reader . Molecular Devices Software. Details. Monday to Friday 7am - 5pm Pacific Time. Monday to Friday 8am - 5pm GMT. The cuvette must be in place when you read the plate. Page 1 of 8 . Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. ORDER. These systems are hybrids that allow you to choose between. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Exploring misconceptions and understanding the technologies. SpectraMax® Microplate Readers and SoftMax® Pro Software. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 5 µL of each sample was pipetted into a 96-well. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. in steps of 0. Microplate Reader Comparison Charts. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. #Flex3) Methods. 0200-6262. Published Dec 16, 2020 | Updated Dec 16, 2020. ABS Test results. The computer controlling the plate reader runs Windows XP. Gemini EM Microplate Readers Using the BD Biosciences Proteasome Sensor Jinfang Liao and Evelyn McGown Molecular Devices Corporation, 1311 Orleans Dr. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. We would like to show you a description here but the site won’t allow us. Vmax® plate reader specifications. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. FlexStation® 3 Multi-Mode Microplate Reader. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. The whole plate can be detect in a single run with ease and flexible. Three low volume 24-well bottom slides. to that of dedicated single-mode readers. Shop now. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. SpectraMax Microplate Readers. Molecular Devices’ AquaMax ® 4000 Microplate Washer aspirates. 304 signal level saturation. . The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 0. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Fluorescence Microplate Reader Comparison Chart. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. One plate carrier adapter is provided with the instrument. Microplate Reader Shelf. FlexStation 3Multi-Mode Microplate Reader (Molecular Devices cat. Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Multi-mode Plate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The adapter is required for optimum performa nce with standard 96-well and 384-. 0. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Tests every subsystem of the reader . 1-10 sec. 9000-0756. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 1. Recertification service. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. 9. After reading the ELISA plate on both readers, each standard curve was nearly identical. 6 % of reading 4. 190. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. 2. The CellTiter-Glo assay from Promega uses luciferase. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. For a higher . Narrow bandwidths deliver increased accuracy and enhanced peak resolution. HTRF-compatible SpectraMax Multi-Mode Readers. BMG Labtech - THERMOstar. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Microplate Reader Shelf. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Step 1: Turn on your microplate reader. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 2 only) SpectraMax M-series Microplate readers. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). How to import latest protocol revisions into SoftMax Pro Protocol Library; Protocols for the ABS2 Validation Plate. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. FlexStation™ II Systems provide integrated fluid transfer capability from a source platePlate Reader SpectraMax iD5 multi-mode system Plate Washer AquaMax 4000 Microplate Washer Robot PreciseFlex 400 Sample Handler Plate Storage Plate Hotels – 40 microtiter plates Controlling Software Genera Control Software Incubation LiCONiC STX44 Automated CO 2 Incubator Liquid Handler Microlab STAR Automated Liquid Handling The. No accessories are needed and SoftMax® Pro Software. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Recombinant protein expression Nucleocapsid. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for . Fluorescence Microplate Reader Comparison Chart. SoftMax® Pro 7. Products : General, FilterMax F5 reader. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software Check software compatibility with Windows 10 and locate your software installer. VIS absorbance detection. Citations on Google Scholar for Molecular. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Uncontrolled Copy for Training and Reference Purposes Only . UV/Vis spectrophotometer & microplate reader with built-in cuvette port. SoftMax® Pro 7. 5. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. Timothy Bolus, MPM, MBA, Compliance Program Manager. This multi plate reader is excellent to work with for checking the U. Luminescence is the emission of light by a substance as a result of a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence). 340 PC384. Also refer to the Multimode Reader Comparison Chart Features. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. 9. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. A control well was set up to verify the accuracy of the standard curve. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. 313 reference gain check fail. ELISA Plate Readers / Microplate Readers. 0077 OD 0. 5577 UK +44. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. 1. 0) OD (0. We would like to show you a description here but the site won’t allow us. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of. Data acquisition and analysis. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. In the M2, M5 and Plus. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The reader measures both flat and round 96-well microplates and. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 1 second up to 30 seconds. San Jose, California, June 5, 2018. Find our technical support information along with contact details. Cell viability는 세포막 Integrity 또는. Select Update Driver. 58% for the detection module and 2. Page 17 Chapter 2: Setting Up the Instrument 4. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 2. 0) OD (500 to 1100) RFU (5. Important things to consider when purchasing a multimode microplate reader. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Published Dec 16, 2020 | Updated Nov 08, 2023. With various on-board features and. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. The plate was incubated at room temperature for 50 minutes or 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. Or, with the reader specifications noted from the Instrument. FlexStation 3 Multimode Microplate Reader (Molecular Devices Cat. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 2. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. The luminescent reaction must be very intense for a reliable reading. Every step is optimized to simplify analysis and reporting to support our microplate readers. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. A. Testing can be done in the user’s lab on their own schedule. Absorbance Plate Reader Comparison Chart. D. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Uncontrolled Copy for Training and Reference Purposes Only . The adapter is required for optimum performance with standard 96-well and 384-. VMax . These plate readers in the list below, are no longer included with the new SoftMax Pro 6 reader menu. Cellular Imaging Systems. you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 1-1. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. coli stock with an OD 600 value of 0. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. B. These robust readers have been placed in labs from. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. Molecular Devices i3x. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Lab automation for high-throughput plate-based assays. #6590-0118) Filter 665/10 (Molecular Devices cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Load up the plates that you need to run and walk away. We would like to show you a description here but the site won’t allow us. Published Dec 16, 2020 | Updated Dec 16, 2020. adipocytes: insulin dose-response. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. ®ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Microplate Reader, Multimode Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Read height should be optimized forIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Absorbance Plate Reader Comparison Chart. #655096) • Brown or amber (light-blocking microcentrifuge tubes • Molecular Devices microplate reader with fluorescence detection mode:What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Multimode Microplate Readers. Fluorescence Microplate Reader Comparison Chart. Imaging System Giles Scientific Biomic V3 Automatic Plate. #0200-7011) Instrument setup GxP regulated industry assessments of microplate readers. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. 1. This document is provided to customers who have purchased Molecular Devices, Inc. 0. Molecular Devices' microplate readers. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Molecular Devices' microplate readers. The adapter is required for optimum performance with standard 96-well and 384-. An industry-renowned instrument for monitoring GPCRs and ion channels. Use the 1. 0 . Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableThe SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. With SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Versa. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. Turn the reader on and secure the USB cable. 561. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Control shake mode, duration, and intensity for a variety of plate formats. If it looks fine, then proceed. White plates reflect the light, enabling even low levels of Luminescence to be detected. 25 to 3. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Tests every subsystem of the reader or . SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. 118. FLIPR Calcium 4 Assay Kit. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The SpectraMax 190 reader can be integrated with Molecular . The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Use all black plate. 7°C (gradient heating design). Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. PURPOSE. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 020 OD 2. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Microplate Readers. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Luminescence reading is a secondary mode of the Gemini instruments. or 0. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. . Absorbance microplate readers from Molecular Devices are used to detect the absorbance in the melamine assay. The adapter is required for optimum performa nce with standard 96-well and 384-. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Instrument settings can be saved as a protocol file. Cell Cytotoxicity assay procedureMolecular Devices. To interrupt the kinetic measurement, the. sensitivity or performance. Microscopes and Microplate Instrumentation. Other features and benefits include: Eliminate changing out filters. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 0112-0127C 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 9 ComputerIntegration 11 InstrumentCovers 11 FluidicsModule 12Plate reader instructions Computer. The guide also covers the software features, the specifications, and the accessories of the systems. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Reader Documentation Version No. Step 4: Incubate at room temperature for 1 hour, protected from light. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. The drawing shows a standard microplate of 15 mm height covered with a lid. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 2. Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. One plate carrier adapter is provided with the instrument. (b) Fluorescence responses of probe HADP and control compounds. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. S.